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Friday, June 3

  1. page Fish Stuff edited Zebrafish and Medaka Protocol for Raising Zebrafish {1605 UPDATE Protocol for raising zebrafis…

    Zebrafish and Medaka
    Protocol for Raising Zebrafish {1605 UPDATE Protocol for raising zebrafish.pdf}
    Techniques and Tools
    Cryosectioning Protocol Cryosectioning {160228 Cryostst Sectioning Protocol.pdf}
    (view changes)
    3:09 pm

Sunday, March 6

  1. page Fish Stuff edited ... Immunohistochemistry Protocol for sections IHC {160301 IHC Protocol.pdf} General Maintenanc…
    ...
    Immunohistochemistry Protocol for sections IHC {160301 IHC Protocol.pdf}
    General Maintenance
    Fish Maintenance
    Embryo Maintenance
    Rotifer Cultures
    Brine Shrimp Cultures

    Medaka Embryo
    Dissection of the Medaka Brain
    (view changes)
    5:08 pm
  2. page Fish Stuff edited ... Zebrafish and Medaka Techniques and Tools Cryosectioning Protocol Cryosectioning {160228 C…
    ...
    Zebrafish and Medaka
    Techniques and Tools
    Cryosectioning Protocol Cryosectioning {160228 Cryostst Sectioning Protocol.pdf}
    Immunohistochemistry Protocol for sections IHC {160301 IHC Protocol.pdf}

    General Maintenance
    Fish Maintenance
    (view changes)
    5:07 pm
  3. 5:07 pm

Monday, December 21

  1. page BPA affects GnRH3 development and locomotion edited Impact of bisphenol A on the developing GnRH3 neural system and locomotor behavior in Japanese me…

    Impact of bisphenol A on the developing GnRH3 neural system and locomotor behavior in Japanese medaka
    Published: Neurotoxicology 10.1016/j.neuro.2015.12.003
    Inagaki, T., Smith, N.*, Lee, E.K.*, Ramakrishnan, S., Low dose exposure to Bisphenol A alters development of Gonadotropin-Releasing Hormone3 neurons and larval locomotor behavior in Japanese Medaka, Neurotoxicology,2015 10.1016/j.neuro.2015.12.003

    *T. INAGAKI1,2, E. K. LEE2, N.L. SMITH, S. RAMAKRISHNAN1,2; 1Biol., 2Neurosci. program, Univ. of Puget Sound, Tacoma, WA
    Abstract:
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    10:29 pm
  2. page Cortisol Effects on GnRH neurons edited Cortisol effects on GnRH neuron development We investigate the effects of chronic stress on the…

    Cortisol effects on GnRH neuron development
    We investigate the effects of chronic stress on the development of GnRH neurons in the early zebrafish (Danio rerio) embryo. Embryonic exposure to stress has been shown to cause problems in the development of GnRH neurons in other fish species most notably salmon and carp. Exposure to stress in zebrafish had been shown to cause problems in reproductive behavior and development. Prior research has also linked abnormal development of the GnRH system in zebrafish to problems in reproduction and reproductive behavior. However, little research has been done on GnRH neuron development under stress in zebrafish. We primarily focused on the extrahypothalamic GnRH1 neurons located in the terminal nerve (TN), with a putative role in neruomodulation and social regulation of reproduction. This study investigates effects of early, chronic cortisol exposure on the TN-GnRH1 neurons of embryonic zebrafish at 2 and 3 days post fertilization (dpf).
    Transgenic zebrafish embryos with GnRH1 neurons tagged with green fluorescent protein (GFP) were used to easily visualize embryonic changes to the developing reproductive neuroendocrine system. Embryos were exposed to a chronic dose of the stress hormone cortisol (hydrocortisone 10μM) by way of waterborne exposure in their embryo medium from 2 hours post fertilization (hpf) through 72 hpf. Embryos were fixed at 48 and 72 hpf, mounted in agar and their TN-GnRH1 neurons were examined using an upright epifluorescent microscope. The perimeters of individual neurons were marked in ImageJ and neuron area (in μm2) and the short-diameter of the fitted ellipse (in μm) were calculated blind.
    Blind analysis of the images showed that embryos exposed to cortisol through 48 hpf (n=37 from 6 fish) showed a significant 23.50% reduction in the area of the individual neurons and a significant 13.54% reduction in the radius of the TN-GnRH1neurons as compared to the control embryos (n=22 from 5 fish). Embryos exposed to cortisol through 3 dpf (n=36 from 7 fish) also showed a significant reduction, but to a lesser degree, of 10.80% in the area and 8.58% in the radius of individual GnRH1 neurons as compared to the control embryos (n=27 from 5 fish). Our study suggests that chronic exposure to stress in the early embryo delays or disrupts the development of TN-GnRH1 neurons in zebrafish (Supported by NSF CAREER Award 1253126 to SR and Univ Puget Sound UEC Grant SR1563 to DS).

    (view changes)
    10:27 pm

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